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本文研究了钴(Ⅲ)-1-(2-吡啶偶氮)-2-萘酚(PAN)络合物的胶束薄层色谱行为,探讨了该络合物的胶束色谱分离机理。以聚酰胺薄片为固定相,6.4％SDS:10％Triton X-100:HAc-NaAc(2.5∶4∶1.5)体系为展开剂,建立了选择性好、灵敏度高的钴的胶束薄层色谱扫描测定法,测定波长为450nm。标准曲线的线性范围为7.5～75纳克/斑点,最小检出量为2.5ng/斑点。方法用于Ni~(2+)、Cu~(2+)等八种金属离子混合液及维生素B~(12)针剂中钴的测定,结果令人满意。
In this paper, the micellar thin-layer chromatography behavior of cobalt (Ⅲ) -1-pyridinium-azo-2-naphthol (PAN) complex has been studied and the mechanism of micellar chromatography separation has been discussed. A sensitive and selective cobalt micellar thin layer chromatography (TLC) was developed using polyamide sheet as the stationary phase and 6.4% SDS: 10% Triton X-100: HAc-NaAc Scanning assay, measuring wavelength 450nm. The standard curve has a linear range of 7.5-75 ng / spot and a minimum of 2.5 ng / spot. The method was applied to the determination of cobalt in eight metal ions such as Ni ~ (2 +) and Cu ~ (2+) and vitamin B ~ (12) injections. The results were satisfactory.