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目的:分析明水县不同采收期柴胡多糖含量动态积累规律。方法:采用蒽酮-硫酸比色法,以葡萄糖作为为对照品,在620nm波长下测定吸光度,分别测定可溶性多糖和粗多糖的含量。结果:在0.0216mg~0.108mg范围内,葡萄糖质量浓度与吸光度值线性关系良好,R2=0.9990。不同采收期的柴胡的总多糖含量不同,随着采收期的延后,总多糖的变化趋势是先下降后上升再下降再上升的规律。其中2号样品(6月30日采收)和6号样品(8月30日采收)的总多糖含量比较低,分别是8.67%和7.49%,3号样品(7月15日采收)和8号样品(10月2日采收)的总多糖含量比较高,分别是12.06%和11.23%。结论:该方法简便、快速、可靠、易行,适合柴胡多糖的含量测定。由于柴胡中其主要有效成分为柴胡皂苷、多糖、植物甾醇、挥发油等,因此多糖含量的多少是评价其质量的一个指标,同时应结合柴胡皂苷含量来评价药材质量,确定最佳采收期。
Objective: To analyze the dynamic accumulation of polysaccharide content of Bupleurum chinense in different harvest periods in Mingshui County. Methods: Using anthrone-sulfuric acid colorimetric method, glucose was used as the reference substance, absorbance was measured at 620nm wavelength, and the contents of soluble polysaccharide and crude polysaccharide were determined. Results: In the range of 0.0216mg~0.108mg, the linear relationship between glucose concentration and absorbance was good, R2=0.9990. The total polysaccharide content of Radix Bupleuri in different harvest periods was different. With the delay of the harvest period, the change trend of the total polysaccharide was first to fall, then rise, then fall and then rise again. The total polysaccharide content of No. 2 sample (collected on June 30th) and No. 6 sample (collected on August 30th) was relatively low, which was 8.67% and 7.49%, respectively, and No. 3 sample (harvested on July 15th). The total polysaccharide content of sample No. 8 (acquired on October 2) was relatively high, which was 12.06% and 11.23%, respectively. Conclusion: This method is simple, rapid, reliable, and easy to implement. It is suitable for determination of polysaccharides in Bupleurum chinense. Since the main effective components of Radix Bupleuri are saikosaponins, polysaccharides, phytosterols, volatile oils, etc., the amount of polysaccharides is an indicator of the quality of the evaluation. At the same time, the quality of medicinal materials should be evaluated by combining saikosaponin content to determine the optimal harvest period. .