Inhibitive effect of triptolide on invasiveness of human fibrosarcoma cells by downregulating matrix

• 作 者: Shengbo Yang Can Gu Guiying Zhang Jian Kang Haiquan Wen QianJin Lu Jinhua Huang
• 机 构: Department of Dermatology,Third Xiangya Hospital,Central South University,School of Nursing,Central
• 出 处: Asian Pacific Journal of Tropical Medicine
• 发表日期: 2011年06期
• 关键词: triptolide inhibited antitumor Tripterygium bioactive invasion doses methylation

Objective:To explore the molecular mechanisms of antitumor properties of triptolide,a bioactive component isolated from the Chinese herb Tripterygium wolfordii Hook F.Methods:Human fibrosarcoma HT-1080 cells were treated with different doses of triptolide for 72 h.Then the expression and activity of matrix metalloproteinase(MMP)-2 and -9 were measured and the invasiveness of triptolide-treated HT-1080 cells was compared with that of anti-MMP-9- treated HT-1080 cells.Results:18 nmol/L triptolide inhibited the gene expression and activity of MMP-9,but not those of MMP-2,in HT-1080 cells.In addition,both 18 nmol/L triptolide and 3μg/mL anti-MMP-9 significantly reduced the invasive potential of HT-1080 cells,by about 50%and 35%, respectively,compared with the control.Whereas there was no significant difference between the effect of 18 nmol/L triptolide and that of anti-MMP-9 on invasive potential of HT-1080 cells. Conclusions:These data suggest that triptolide inhibits tumor cell invasion partly by reducing MMP-9 gene expression and activity.

Objective: To explore the molecular mechanisms of antitumor properties of triptolide, a bioactive component isolated from the Chinese herb Tripterygium wolfordii Hook F. Methods: Human fibrosarcoma HT-1080 cells were treated with different doses of triptolide for 72 h. The expression and activity of matrix metalloproteinase (MMP) -2 and -9 were measured and the invasiveness of triptolide-treated HT-1080 cells was compared with that of anti-MMP-9-treated HT-1080 cells. Results: 18 nmol / L triptolide inhibited the gene expression and activity of MMP-9, but not those of MMP-2, in HT-1080 cells. In addition, both 18 nmol / L triptolide and 3 μg / mL anti- MMP- 9 significantly reduced the invasive potential of HT- 1080 cells, by about 50% and 35%, respectively, compared with the control. There was no significant difference between the effect of 18 nmol / L triptolide and that of anti-MMP-9 on invasive potential of HT-1080 cells. Conclusions : These data suggest that triptolide inhibits tumor cell invasion partly by reducing MMP-9 gene expression and activity.