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目的 探讨腺病毒介导的野生型p53在前列腺癌基因治疗中的可能性及其机制。方法培养前列腺癌细胞系PC3M,转染腺病毒分导的野生型p53,检测其增殖和凋亡水平变化,并检测其bax蛋白及mRNA水平。结果 腺病毒介导的野生型p53可高水平地转染人PC3M细胞,转染后的PC3M细胞增殖受到抑制、凋亡水平增高,细胞中bax蛋白及mRNA水平皆增高。结论 腺病毒介导的野生型p53可抑制PC3M细胞增殖并促进其细胞凋亡。其促进凋亡的机制可能是通过促进bax表达而实现的。
Objective To investigate the possibility and mechanism of adenovirus-mediated wild-type p53 in gene therapy of prostate cancer. Methods Prostate cancer cell line PC3M was cultured and transfected with adenovirus-directed wild-type p53. Proliferation and apoptosis were detected and the protein and mRNA levels of bax were detected. Results Adenovirus-mediated wild-type p53 could be transfected into human PC3M cells at high level. The proliferation of PC3M cells after transfection was inhibited and the level of apoptosis increased. The expression of bax protein and mRNA in the cells increased. Conclusion Adenovirus-mediated wild-type p53 can inhibit PC3M cell proliferation and promote its apoptosis. Its mechanism of promoting apoptosis may be achieved through the promotion of bax expression.