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目的探讨优选处理前和处理后精子顶体酶活性的变化,及与体外受精(IVF)受精率的相关性。方法采用分光光度比色法,对接受IVF治疗的53例不育夫妇男方精液,分别测定优选处理前和处理后精子顶体酶活性,分析其与IVF受精率的相关性。结果优选处理后精子顶体酶活性与优选前比较有显著性差异(P<0.05);达到常规IVF标准并选择常规IVF治疗者,优选后的精子顶体酶活性与IVF受精率有相关性,精子顶体酶活性降低与IVF受精率降低有关。结论精子顶体酶活性与IVF受精率有相关性,并且通过精子顶体酶活性可以预测IVF受精率。
Objective To investigate the changes of sperm acrosin activity before and after pretreatment and the correlation with in vitro fertilization (IVF) fertilization rate. Methods Fifty-three male infertile couples received IVF treatment by spectrophotometry. The acrosin activity of pre-treatment and post-treatment sperm were measured respectively. The correlation between IVF fertilization rate and sperm acrosin activity was analyzed. Results The results showed that there was a significant difference (P <0.05) between acrosin activity and sperm acrosin activity after pretreatment. Conventional IVF standard and routine IVF treatment were selected. The sperm acrosin activity was correlated with IVF fertilization rate, Sperm acrosin activity decreased and IVF fertilization rate decreased. Conclusion The sperm acrosin activity is related to IVF fertilization rate, and IVF fertilization rate can be predicted by acrosin activity.