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文献报道,近几年研究证实石刁柏(Asparagus officinalis)具有抗菌、抗突变、利尿、细胞毒、抗病毒和杀螺等活性,石刁柏的根和枝中含有大量的皂甙,是主要的活性成分。本文从石刁柏的种子中分离、鉴定2种甾体皂甙,并研究了它们对人体白血病HL-60细胞中DNA、RNA、蛋白质的合成及细胞生长的作用。 石刁柏种子经脱脂后先用正己烷提取3次,再用甲醇提取5次,收集甲醇提取液,去除溶媒后溶于水中,并依次用乙酸乙酯和正丁醇提取。正丁醇提取液经Diaion HP-20层
According to reports in the literature, studies in recent years have confirmed that Asparagus officinalis has antibacterial, antimutagenic, diuretic, cytotoxic, antiviral and molluscicidal activities. The roots and branches of Asparagus officinalis contain a large amount of saponins, which is the main activity. ingredient. In this paper, two steroidal saponins were isolated and identified from the seeds of Asparagus officinalis and their effects on DNA, RNA, protein synthesis and cell growth in human leukemia HL-60 cells were studied. The seeds of Asparagus officinalis were defatted and extracted with n-hexane three times, and then extracted with methanol five times. The methanol extract was collected, dissolved in water after removing the solvent, and extracted with ethyl acetate and n-butanol in turn. Butanol Extract Via Diaion HP-20 Layer