论文部分内容阅读
目的分析miR-196a2 rs11614913和miR-149 rs2292832单核苷酸多态性与缺血性脑卒中的相关性。方法选取378例缺血性脑卒中患者为病例组,378名经性别和年龄匹配且未患缺血性脑卒中的正常体检者为对照组,收集两组一般体检资料并采用Taqman-PCR和DNA直接测序技术检测两组miR-196a2 rs11614913和miR-149 rs2292832位点基因型和等位基因频率,采用Gen AIEx 6.5软件进行哈代-温伯尔遗传平衡定律分析,采用条件Logistic回归分析上述位点与缺血性脑卒中发病风险的关系。结果病例组患有糖尿病、高血压、体质指数≥24 kg/m2及血脂代谢异常患者比例均高于对照组(P<0.01)。miR-196a2 rs11614913位点等位基因C、基因型TC和CC与缺血性脑卒中均无统计学关联(P>0.05),miR-149 rs2292832与缺血性脑卒中在等位基因模型(C vs.T:P=0.026,OR=1.269,95%CI=1.031~1.569)、共显模型(CC vs.TT:P=0.011,OR=1.812,95%CI=1.150~2.850)和隐性模型(CC vs.TC+TT:P=0.008,OR=1.739,95%CI=1.142~2.647)与缺血性脑卒中相关。结论 miR-149 rs2292832位点与缺血性脑卒中相关,提示此位点可能参与缺血性脑卒中的发病过程,是遗传风险易感位点。
Objective To analyze the association of miR-196a2 rs11614913 and miR-149 rs2292832 single nucleotide polymorphisms with ischemic stroke. Methods A total of 378 ischemic stroke patients were selected as the case group and 378 normal subjects without sex and age matched for ischemic stroke were selected as the control group. Two groups of general physical examination data were collected and analyzed by Taqman-PCR and DNA The genotype and allele frequencies of miR-196a2 rs11614913 and miR-149 rs2292832 loci were detected by direct sequencing. Gen AIEx 6.5 software was used to analyze the Hardy-Wimbler genetic balance, and conditional Logistic regression was used to analyze the association between these loci and Relationship between ischemic stroke and risk of ischemic stroke. Results The cases of patients with diabetes, hypertension, body mass index ≥ 24 kg / m2 and dyslipidemia patients were higher than the control group (P <0.01). miR-196a2 rs11614913 allele C, genotypes TC and CC were not associated with ischemic stroke (P> 0.05). miR-149 rs2292832 was associated with ischemic stroke in the allele model (C (CC vs. TT: P = 0.011, OR = 1.812, 95% CI = 1.150 to 2.850) and recessive model (vs.T: P = 0.026, OR = 1.269, 95% CI = 1.031 to 1.569) (CC vs. TC + TT: P = 0.008, OR = 1.739, 95% CI = 1.142-2.657) were associated with ischemic stroke. Conclusions The miR-149 rs2292832 locus is associated with ischemic stroke, suggesting that this locus may be involved in the pathogenesis of ischemic stroke and is a predisposing locus for genetic risk.