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目的:探讨E-选择素(E-selectin)基因第2号外显子G98T和第4号外显子A561C多态性及血浆可溶性水平与儿童慢性HBV感染的相关性。方法:采用聚合酶链反应-限制性片段长度多态性分析技术(PCR-RFLP)检测119例慢性HBV感染患儿和141例健康对照者E-选择素基因多态性,同时采用酶联免疫吸附实验(ELISA)检测各组可溶性E-选择素(sE-选择素)水平。结果:血浆sE-选择素水平在慢性HBV感染组与对照组相比差异有统计学意义(P<0.01);E-选择素G98T和A561C多态性中基因型频率和等位基因频率在慢性HBV组与对照组相比差异无统计学意义,各组中C等位基因携带者血浆sE-选择素水平明显高于A等位基因携带者(P<0.05)。结论:E-选择素G98T和A561C基因多态性在慢性HBV感染中不起作用,但A561C基因多态性参与调控血浆可溶性E-选择素的表达。
Objective: To investigate the association between E-selectin gene exon 2 G98T and exon 4 A561C polymorphism and plasma soluble level in children with chronic HBV infection. Methods: E-selectin gene polymorphism was detected in 119 children with chronic HBV infection and 141 healthy controls by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Enzyme-linked immunosorbent assay Adsorption assay (ELISA) was used to detect soluble E-selectin (sE-selectin) levels in each group. Results: The plasma levels of sE-selectin were significantly different from those in the control group (P <0.01). The frequencies of genotypes and alleles in the E-selectin G98T and A561C polymorphisms were significantly different between the chronic There was no significant difference between HBV group and control group. The plasma levels of sE-selectin in C allele carriers were significantly higher than those in A allele carriers (P <0.05). CONCLUSION: E-selectin G98T and A561C polymorphisms are not effective in chronic HBV infection, but A561C polymorphism is involved in the regulation of plasma soluble E-selectin expression.