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通过ＤＮＡ重组技术，将不含非编码区的人粒细胞集落刺激因子（ｈＧ－ＣＳＦ）ｃＤＮＡ重组入逆转录病毒质粒ｐＬＸＳＮ中。重组质粒转染ＰＡ３１７细胞后，经Ｇ４１８筛选，抗性克隆细胞培养上清液能成功地感染ＮＩＨ３Ｔ３细胞，使之在筛选培养基中形成典型的Ｇ４１８抗性克隆。该克隆细胞染色体中成功地整合了ｈＧ－ＣＳＦｃＤＮＡ，并且表达了有生物学活性的ｈＧ－ＣＳＦ产物。
The human granulocyte colony-stimulating factor (hG-CSF) cDNA, which contains no non-coding region, was recombined into the retroviral plasmid pLXSN by DNA recombination technology. The recombinant plasmid was transfected into PA317 cells and then screened by G418. The resistant clone cell culture supernatant could successfully infect NIH3T3 cells to form typical G418 resistant clones in the screening medium. The hG-CSF cDNA was successfully integrated into the chromosome of the cloned cell and the biologically active hG-CSF product was expressed.