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目的建立一种实用、标准、可靠的急性大鼠脊髓损伤(SCI)模型,并对其稳定性进行评价。方法将20只SD大鼠随机均分为SCI组和对照组。SCI组打开T_8~T_(10)节段脊髓,暴露硬脊膜,在T_9水平用Leica Impact One打击器打击,力度设定为0.5m/s,以鼠尾痉挛性摆动并下垂、双下肢软瘫为模型成功标准;对照组只暴露硬脊膜。分别于造模前及造模后各时间点进行两组双后肢BBB运动功能评分。结果造模成功率100%。SCI组于造模第1周开始恢复,观察至造模后第4周基本停止,造模后各时间点SCI组大鼠BBB运动功能评分均低于对照组(P<0.05)。脊髓HE染色及电镜结果符合SCI的病理学变化,且死亡率相对较低。结论采用Leica Impact One打击器建立的急性SCI模型具有建模方法简便、稳定性高、死亡率低的优点。
Objective To establish a practical, standard and reliable acute rat model of spinal cord injury (SCI) and evaluate its stability. Methods Twenty SD rats were randomly divided into SCI group and control group. The spinal cord of T_8 ~ T_ (10) segment was opened in SCI group, the dura was exposed, and hit with a Leica Impact One percussion instrument at the T_9 level. The intensity was set at 0.5 m / s, with a squirming tail twitching and drooping. Paralysis as a model of success criteria; control group only exposed dura. The BBB motor function scores of two groups of hind limbs were measured before modeling and at various time points after modeling. Results model success rate of 100%. SCI group began to recover from the first week of modeling. The rats in the SCI group were stopped basically 4 weeks after modeling. BBB motor function score of SCI group was lower than that of the control group (P <0.05). Spinal cord HE staining and electron microscopy results consistent with the pathological changes of SCI, and the mortality rate is relatively low. Conclusion The acute SCI model established by Leica Impact One Impactor has the advantages of simple modeling method, high stability and low mortality rate.