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目的:揭示影响肿节风药材反丁烯二酸含量的因素,为肿节风的开发利用和质量评价提供依据。方法:建立反丁烯二酸的HPLC测定方法,并测定不同生长方式、生长年限、采收期、部位以及不同产地肿节风药材中反丁烯二酸的含量。结果:5个因素对肿节风反丁烯二酸的含量都有影响。栽培品比野生品高;3年生比2年和1年生的高;10月份采收的药材含量最高;茎、叶、根、果实中的含量依次降低;广东和浙江龙泉药材含量明显高于其它9个产地的药材。结论:本研究建立的肿节风反丁烯二酸含量测定方法简便、准确、重现性好,可用于肿节风药材中该成分的质量控制。本研究结果为肿节风的开发利用和质量评价提供了一定依据。
OBJECTIVE: To reveal the factors affecting the content of fumaric acid in swollen wind herbs and provide basis for the development, utilization and quality evaluation of swollen-stem-wind. Methods: The HPLC method for the determination of fumaric acid was established and the content of fumaric acid was determined in different growth modes, growth period, harvesting period, site and the different producing sites. Results: Five factors had an effect on the content of fumaric acid. The cultivars were higher than the wild ones; the 3-year-olds were higher than those of 2-year and 1-year-olds; the content of medicinal materials was the highest in October; the content of stems, leaves, roots and fruits decreased successively; the contents of herbs in Guangdong and Zhejiang were significantly higher than other 9 origin of herbs. Conclusion: The method for the determination of fumaric acid fumaric acid in this study is simple, accurate and reproducible. It can be used for the quality control of the components in Fengjiefeng medicinal herbs. The results of this study provide a certain basis for the development and utilization of the typhoid fever and the quality evaluation.