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为人工调控花色苷合成,以‘京优’葡萄为实验材料,应用液相色谱-质谱(HPLC-MS)技术和荧光定量PCR,研究了萘乙酸(NAA)和脱落酸(ABA)处理对葡萄果皮花色苷积累及其生物合成相关基因表达的影响。结果表明:在‘京优’葡萄果皮中,可检测到16种花色苷;ABA处理的花色苷含量高于对照,NAA处理低于对照,并且ABA处理的果皮比对照和NAA多出3种花色苷;葡萄果实发育进入转色期,花色苷合成过程中类黄酮途径的结构基因上调表达,完熟后下调表达;ABA处理的果皮花色苷结构基因相对表达量高于对照,而NAA则低于对照。外源ABA促进花色苷积累及其合成相关基因的表达,而NAA则抑制其表达。
In order to artificially control the anthocyanin synthesis, we studied the effects of NAA and ABA treatment on the growth of grape by using Jingyou grape as experimental material and HPLC-MS and fluorescence quantitative PCR. Effects of Anthocyanin Accumulation and Biosynthesis Related Genes Expression in Pericarp. The results showed that 16 kinds of anthocyanins could be detected in the peel of ’Jingyou’ grape. The content of anthocyanin in ABA treatment was higher than that in the control, NAA treatment was lower than that in the control, and ABA treatment had 3 kinds of flower color The results showed that the expression of anthocyanin structural gene in ABA-treated peel was higher than that in control, but NAA was lower than that in control . Exogenous ABA promoted anthocyanin accumulation and the expression of genes involved in its synthesis, whereas NAA inhibited its expression.