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突变的诱发受许多因素影响,诸如品种(系)、细胞类型、代谢状态和修复过程等。体细胞突变作为诱变处理或自发突变的结果,发生在已分化的细胞中。而 M_2代观察到的隐性突变才是真正的遗传突变,可通过配子细胞传给后代。然而诱发的体细胞突变和遗传突变呈正相关。因此诱变剂诱发的遗传突变可以由测定 M_1代的体细胞突变来估计。本试验以大豆为材料,研究了 EMS 诱发的 M_1代植株体细胞突变和 M_2代群体叶绿素缺失突变的分离,并用~(14)C 标记的 EMS 对其在种子组织中的分布作了示踪研究。
Mutation induction is affected by many factors, such as variety (line), cell type, metabolic status and repair process. Somatic mutations occur as mutagenized or spontaneous mutations in differentiated cells. The recessive mutation observed in M_2 generation is the true genetic mutation, which can be passed to the offspring through the gametocyte. However, somatic mutations and genetic mutations were positively correlated. Therefore mutagen-induced genetic mutations can be estimated by determining somatic mutations in the M-1 generation. In this experiment, the somatic cell mutation of M 1 plants induced by EMS and the chlorophyll deletion mutation of M 2 population were studied using soybean as material, and their distribution in seed tissues was traced by ~ (14) C-labeled EMS. .