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本文通过测定荧光标记6-羧基荧光素(CF),比较包裹在中性双肉豆蔻酰磷脂酰胆碱(DMPC)/胆固醇(分子比1∶1)和负电荷DMPC/胆固醇/联十六烷基磷酸酯(DCP)(分子比1∶1∶0.2)脂质体中水相标记物的肺生物利用度。脂质体采用薄膜法制备,以0.25mol/L CF水溶液水化,再用超声波将多层脂质体分散制成以上两种脂质体,用凝胶层析和超滤除去未包裹的CF,平均粒径分别
In this study, we measured the fluorescence of labeled 6-carboxyfluorescein (CF) and compared the binding capacity of neutral dimyristoylphosphatidylcholine (DMPC) / cholesterol (1: 1) and negatively charged DMPC / cholesterol / Phosphates (DCP) (Molecular ratio 1: 1: 0.2) Pulmonary bioavailability of aqueous phase markers in liposomes. The liposomes were prepared by the membrane method, hydrated with 0.25mol / L CF aqueous solution, and then the multilamellar liposomes were dispersed by ultrasound to make the above two liposomes. The unfolded CFs were removed by gel filtration and ultrafiltration , The average particle size respectively