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关于双氢链霉素的耳毒性已早有记载,但其抵达内耳之途径至今尚未查明。为此,著者进行氚化(tritiated)双氢链霉素在豚鼠耳蜗分布的自动放射照像术(autoradiographic)研究。实验材料及方法:取双氢链霉素硫酸盐200毫克溶于含有10居里氚气及25毫克预先还原了的铂催化剂的0.3毫升水中,室温下磁力搅拌过夜,其中不稳定的氚通过10毫升水将其去除(在真空下蒸馏),这种标记好的混合物的能量是0.38和0.26毫居里/毫克。以体重200—250克的年青豚鼠为实验对象,以20—30毫居里/公斤体重的氚化双氢链霉素硫酸盐注射入8只豚鼠腹腔,在乙
The ototoxicity of dihydrostreptomycin has long been documented, but its route to the inner ear has not yet been identified. To do this, the authors conducted autoradiographic studies of cochlear distribution of tritiated dihydrostreptomycin in guinea pigs. Experimental Materials and Methods: 200 mg of dihydrostreptomycin sulfate was dissolved in 0.3 ml of water containing 10% of tritium gas and 25 mg of the prereduced platinum catalyst and stirred magnetically overnight at room temperature with unstable tritium passed through 10 It was removed by a few milliliters of water (distilled under vacuum) and the energy of this labeled mixture was 0.38 and 0.26 millicuries per milligram. Taking 200-250 g of young guinea pigs as experimental subjects, tritiated dihydrostreptomycin sulfate in an amount of 20-30 millicuries / kg body weight was injected into the peritoneal cavity of 8 guinea pigs,