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目的 建立同时测定血清中异烟肼 (INH)、利福平(RFP)、吡嗪酰胺 (PZA)浓度的反相高效液相色谱法。方法 RFP检测条件 :SymmetryC8色谱柱 (5 μm ,15 0mm× 3 9mm) ,流动相为磷酸盐缓冲液 (pH 5 5 )∶甲醇 =30∶70 ,流速0 4 5ml/min ,测定波长 340nm ;INH、PZA检测条件 :Sym metryC18色谱柱 (5 μm ,2 5 0mm× 4 6mm) ,流动相为磷酸盐缓冲液 (pH 6 0 )∶乙腈 =91∶9,流速 0 8ml/min ,测定波长2 5 4nm。结果 以峰高外标法定量 ,线性范围 :利福平为0 5～ 32mg/L ,INH为 0 5～ 2 0mg/L ,PZA为 1 0～ 6 0 0mg/L ,均具有良好的线性关系。 3种药物的日内、日间变异均 <10 %。回收率 >80 %。结论 该方法简便、快速 ,灵敏度和准确度较高 ,无干扰 ,用于上述 3种药物复合制剂卫非特片的药代动力学研究 ,证明能满足人体血药浓度监测及药代动力学研究的需要
Objective To establish a reverse-phase high-performance liquid chromatography (HPLC) method for the simultaneous determination of INH, RFP and PZA in serum. Methods RFP detection conditions: SymmetryC8 column (5 μm, 150 mm × 39 mm), the mobile phase was phosphate buffer (pH 5 5): methanol = 30:70, flow rate of 0 4 5ml / min, , PZA detection conditions: Sym metryC18 column (5 μm, 250 mm × 4 6mm), the mobile phase phosphate buffer (pH 60): acetonitrile = 91: 9, flow rate 0 8ml / min, 4nm. The results were quantitatively determined by peak height external standard method. The linear range was 0.5 ~ 32 mg / L for rifampicin, 0 5 ~ 20 mg / L for INH and 10 ~ 60 mg / L for PZA, all of which had a good linear relationship . The intra-day and inter-day variation of the three drugs were all <10%. Recoveries> 80%. Conclusion The method is simple, rapid, sensitive and accurate, and has no interference. It can be used in the pharmacokinetic studies of the above three kinds of drug combination Wei Wei Te tablets, which proves to be able to meet the requirements of human plasma concentration monitoring and pharmacokinetic studies need