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为探讨外源亚精胺(Spd)对盐碱胁迫下番茄根系线粒体功能的影响,采用水培法,以耐性不同的两个番茄品种‘金棚朝冠’(耐盐型)和‘中杂9号’(敏感型)为试材,通过模拟盐碱生态条件(Na Cl∶Na_2SO_4∶Na HCO3∶Na2CO3=1∶9∶9∶1),结合叶面喷施外源0.25mmol·L~(-1)Spd,研究盐碱胁迫8 d后Spd对番茄幼苗根系形态和根系线粒体功能的影响.结果表明:盐碱胁迫下,两个品种番茄根系线粒体内H_2O_2和丙二醛(MDA)含量增加,线粒体膜通透性明显增大,流动性降低,膜电位、线粒体内细胞色素c/a(Cyt c/a)吸光度比值、膜H~+-ATPase活性显著下降,使线粒体受到不同程度的损伤,从而抑制根系生长,且‘金棚朝冠’的上述指标变化幅度均小于‘中杂9号’.盐碱胁迫下,喷施外源Spd处理的两个品种根系线粒体H_2O_2和MDA含量显著降低,膜通透性减小、流动性增加,膜电位、线粒体内Cyt c/a吸光度比值、膜H~+-ATPase活性显著提高,可有效缓解盐碱胁迫对番茄幼苗根系线粒体的伤害作用,且这种缓解作用在‘中杂9号’上的表现效果更佳.
In order to investigate the effect of exogenous spermidine (Spd) on the mitochondrial function of tomato root under salinity-alkaline stress, two tomato cultivars, ’Jinpengchaguan’ (salt-tolerant) and ’Zhongzhong 9 (sensitive type) was used as test material to simulate the ecological conditions of saline-alkali (Na Cl: Na 2 SO 4: NaHCO 3: Na 2 CO 3 = 1: 9: 9: 1) -1) Spd was used to study the effects of Spd on root morphology and mitochondrial function of tomato seedlings after 8 days of salt-alkali stress.The results showed that mitochondrial H_2O_2 and malondialdehyde (MDA) contents in roots of two tomato cultivars increased under salt-alkali stress , The permeability of mitochondrial membrane increased significantly, the fluidity decreased, the membrane potential, mitochondrial Cyt c / a absorbance ratio and membrane H ~ + -ATPase activity decreased significantly, which caused the mitochondria to be damaged to some extent , And the growth of root system was restrained, and the change range of the above index of ’Jinpengchaoguan’ was less than that of ’Zhongza 9’. The mitochondrial H 2 O 2 and MDA contents in root of two cultivars treated with exogenous Spd were significantly decreased , Membrane permeability decreased, fluidity increased, membrane potential, mitochondrial Cyt c / a absorbance ratio, membrane H ~ + -AT Pase activity was significantly increased, which can effectively mitigate salt-alkali stress on the mitochondria damage of tomato seedling roots, and this mitigation effect in ’Zhongza 9’ better performance.