论文部分内容阅读
本文用小鼠宫颈癌Ｕ_（１４）为模型，研究了不同方法处理的巨噬细胞（Ｍφ）体外细胞毒活性和体内抑瘤效应。结果显示经痘苗病毒瘤苗（ＶＶ－ＴＣＶ）处理的Ｍφ体外细胞毒活性没有明显增加，但ＷＩＮＮ试验显示经ＶＶ－ＴＣＶ和ＴＣＶ匀浆处理的Ｍφ体内抑瘤作用明显高于对照组。腹腔接种Ｕ_（１４）后１３天的各组小鼠脾细胞细胞毒活性结果表明转输ＶＶ－ＴＣＶ处理的Ｍφ组小鼠脾细胞对同一靶细胞的杀伤活性最强。以上结果提示经ＶＶ－ＴＣＶ处理的Ｍφ可能通过其抗原提呈作用，增强了Ｔ细胞的杀瘤作用。而ＶＶ的作用可能与其修饰肿瘤细胞，使肿瘤细胞＂异种化＂，增强其免疫原性有关。
In this paper, the mouse cervical cancer U_ (14) as a model to study the different methods of treatment of macrophages (Mφ) in vitro cytotoxic activity and in vivo anti-tumor effect. The results showed that in vitro cytotoxic activity of Mφ treated with vaccinia virus vaccine (VV-TCV) did not increase significantly. However, WINN assay showed that the anti-tumor effect of Mφ treated with VV-TCV and TCV was significantly higher than that of control. The cytotoxic activity of splenocytes in each group of mice after intraperitoneal inoculation of U_ (14) for 13 days showed that the splenocytes of Mφ-treated MPS mice transfected with VV-TCV had the strongest cytotoxic activity on the same target cells. The above results suggest that Mφ treated with VV-TCV may enhance the killing effect of T cells through its antigen presentation. The role of VV may be related to its modification of tumor cells, tumor cells “heterogeneity” and enhance their immunogenicity.