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目的:比较P-gp和MDR1在人乳腺癌敏感细胞(MCF-7/S)和耐药细胞(MCF-7/ADR、MCF-7/TAM)中的表达差异,初步探讨乳腺癌细胞对阿霉素与对三苯氧胺产生耐药机制的区别。方法:采用免疫细胞化学法、流式细胞术检测P-gp,采用实时荧光定量PCR法检测MDR1在三种乳腺癌细胞中的表达情况。结果:在MCF-7/ADR细胞中P-gp和MDR1均呈高表达,阳性表达率与MCF-7/S细胞比较,有统计学意义(P<0.01)。在MCF-7/TAM细胞中P-gp、MDR1均呈低表达,与MCF-7/S细胞比较,无统计学意义(P>0.05)。结论:P-gp和MDR1的高表达是乳腺癌细胞对阿霉素产生耐药的主要机制,而并非是乳腺癌细胞对三苯氧胺产生耐药的机制。
OBJECTIVE: To compare the expression of P-gp and MDR1 in human breast cancer sensitive cells (MCF-7 / S) and drug-resistant cells (MCF-7 / ADR, MCF-7 / TAM) Differences between mycotoxins and resistance to tamoxifen. Methods: P-gp was detected by immunocytochemistry and flow cytometry. The expression of MDR1 in three kinds of breast cancer cells was detected by real-time fluorescence quantitative PCR. Results: Both P-gp and MDR1 were highly expressed in MCF-7 / ADR cells. The positive expression rate of MCF-7 / S cells was statistically significant (P <0.01). The expression of P-gp and MDR1 in MCF-7 / TAM cells was lower than that in MCF-7 / S cells (P> 0.05). Conclusion: The high expression of P-gp and MDR1 is the main mechanism of resistance to doxorubicin in breast cancer cells, but not the mechanism of breast cancer cell resistance to tamoxifen.