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目的 研究人疱疹病毒 6型 (HHV 6 )感染与淋巴瘤的关系。 方法 用间接免疫荧光试验及聚合酶链反应 ,分别检测淋巴瘤患者和对照组 (其它血液病 )患者血清中抗HHV 6IgG及外周血单个核细胞中的HHV 6DNA序列。用免疫组织化学染色法检测淋巴瘤患者淋巴结组织标本中HHV 6抗原。 结果 淋巴瘤患者抗HHV 6IgG的阳性率为 95 5 % ,几何平均滴度为 1∶12 3,明显高于对照组 (P <0 0 5 )。淋巴瘤患者标本中HHV 6DNA的检出率明显高于对照组 (P <0 0 5 )。免疫组织化学染色法检测的 5例淋巴瘤患者的淋巴结组织标本中 ,4例HHV 6抗原阳性。 结论 HHV 6感染可能与淋巴瘤的发病有关
Objective To investigate the relationship between human herpesvirus 6 (HHV 6) infection and lymphoma. Methods Indirect immunofluorescence assay and polymerase chain reaction were used to detect the HHV 6 DNA sequences of anti-HHV 6 IgG and peripheral blood mononuclear cells from patients with lymphoma and controls (other blood diseases) respectively. Immunohistochemical staining was used to detect HHV 6 antigen in lymph node tissue samples from patients with lymphoma. Results The positive rate of anti-HHV 6 IgG in patients with lymphoma was 95.5%. The geometric mean titer was 1:12 3, which was significantly higher than that of the control group (P <0.05). The detection rate of HHV 6 DNA in patients with lymphoma was significantly higher than that in the control group (P <0.05). Immunohistochemical staining detected in 5 cases of lymphoma in patients with lymph node tissue specimens, 4 cases of HHV 6 antigen positive. Conclusion HHV 6 infection may be related to the incidence of lymphoma