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1986年～1991年间,收集16例NANB型重肝、15例乙型重肝、5例药物性重肝病人的血清,在一80℃下冰冻贮存。这些病人均无近期到戊肝流行区旅行史。使用逆转录PCR方法(60个循环),随后使用intermal探针来检查待检血清中的HEVRNA,通过免疫荧光竞争法和蛋白印迹法研究HEV抗体的存在。结果:16例NANB重肝病人中4例(25%)通过旦白印迹法证实抗HEV阳性,其中1例
Between 1986 and 1991, 16 serum samples of NANB-type, 15-type B and 5-drug-resistant patients were collected and stored frozen at -80 ℃. None of these patients have a recent history of travel to endemic areas of HEV. Reverse transcription PCR (60 cycles) was used followed by the intermal probe to examine HEV RNA in the sera to be tested, and the presence of HEV antibodies was studied by immunofluorescence competition and Western blotting. Results: Four of 16 (25%) NANB patients with severe hepatitis were confirmed positive for anti-HEV by Western blotting and one of them