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酶联免疫印渍法是70年代末80年代初发展起来的新技术,该技术不仅为分子生物学和生物化学研究的重要手段之一,而且用以诊断疾病也具有较好的效果。近年来,该法已成功地用于诊断AIDS病。我们应用这一技术,检测包虫病患者血清31份、健康人31份及囊虫、血吸虫、丝虫、华支睾吸虫、钩虫、肺吸虫、利什曼原虫病患者血清各5份,结果表明,有90％(28/31)包虫病患者血清抗体与羊和人棘球幼囊液中分子量在60KD、36KD、32KD、24KD、20KD、17KD和12KD的抗原组分起反应。尤
The enzyme-linked immunosorbent assay (ELISA), a new technique developed in the late 1970s and early 1980s, is not only one of the most important molecular and biochemical methods, but also has a good effect on the diagnosis of diseases. In recent years, the law has been successfully used to diagnose AIDS. We applied this technique to detect serums from 31 patients with echinococcosis, 31 healthy individuals and 5 serums from patients with cysticercosis, schistosomiasis, filariasis, Clonorchis sinensis, hookworm, paragonimiasis and leishmaniasis, The results showed that 90% (28/31) of the echinococcosis patients serum antibody and sheep and human spicular fluid of molecular weight in the 60KD, 36KD, 32KD, 24KD, 20KD, 17KD and 12KD antigen component reaction. especially