论文部分内容阅读
芳香族化合物适当时间适当浓度添加到培养基中,可提高真菌漆酶活性,有助于增强其对木质纤维素的利用效率。为了增强斑玉蕈漆酶活性,本文研究了8种芳香族化合物对其酶活的影响及其与菌丝生物量的相关性。研究发现在无诱导物条件下,斑玉蕈漆酶活性和菌丝生物量相关系数r为0.9956,说明它们呈正相关,但是整个培养过程漆酶活性相对较低;供试的芳香族化合物对漆酶活性都有不同程度的诱导作用,其中添加0.1mmol/L的愈创木酚对斑玉蕈漆酶活性诱导作用最大,达到3倍以上,同时提高了斑玉蕈菌丝生长速度和菌丝生物量;而随着添加时间的延长,部分化合物对漆酶活性和菌丝生物量都产生不同程度的抑制作用,这可能因为化合物对菌丝毒性的延长导致菌丝生长变慢或死亡;进一步研究发现,斑玉蕈3个漆酶同工酶基因lcc2、lcc3和lcc4在诱导剂愈创木酚的影响下转录水平都不同程度地上调。研究结果表明诱导漆酶活性可以提高斑玉蕈菌丝生长速度和生物量,暗示可能通过提高漆酶活性的方法,提高斑玉蕈的培养基利用效率。
The proper concentration of aromatics to the medium at the right time increases fungal laccase activity and helps to enhance its utilization of lignocellulose. In order to enhance the activity of laccase, the effects of eight aromatic compounds on the enzyme activity and their correlation with mycelial biomass were studied. The results showed that in the absence of inducer, the correlation coefficient between the activity of laccase and the biomass of mycelia was 0.9956, indicating that they were positively correlated, but laccase activity was relatively low during the whole culture process. The tested aromatics on lacquer Enzyme activity had different degrees of induction. The addition of 0.1mmol / L guaiacol had the greatest effect on the activity of laccase, and reached more than 3 times, and at the same time, it increased the mycelia growth rate and mycelium But with the addition of time, some of the compounds had different degrees of inhibition on laccase activity and mycelial biomass, which may be due to the compound hypochlorous toxicity caused by the extension of mycelium growth slowed down or died; further The results showed that the transcriptional levels of three laccase isoenzymes lcc2, lcc3 and lcc4 were up-regulated to varying degrees under the induction of guaiacol. The results showed that the induction of laccase activity can enhance the mycelial growth rate and biomass of A. facilis, suggesting that it is possible to improve the utilization efficiency of P. edodes by increasing the activity of laccase.