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采用HPLC-DAD法,同时测定六味五灵片中连翘酯苷A,特女贞苷,连翘苷,五味子醇甲,五味子酯甲,五味子甲素和五味子乙素共7种有效成分的含量.采用Inertsil(R) ODS-3色谱柱(250 mm×4.6 mm,5 μm),流动相为0.1％磷酸水(A)-乙腈(B),梯度洗脱,柱温25℃,检测波长230 nm.7种化合物在相应的浓度范围内线性关系良好,相关系数均大于0.999.检测限与定量限分别在0.03-0.28 μg/mL和0.11-0.87 μg/mL之间.精密度、稳定性与重复性的RSD均小于2.34％,平均回收率均在96.71％-103.9％之间.建立的含量测定方法可有效对山东世博金都医药有限公司的十批样品进行检测,为六味五灵片的质量控制与下一步药理研究提供科学依据.“,”We employed high performance liquid chromatography (HPLC) method coupled with diode array detector (DAD) for the determination of seven major bioactive compounds (forsythoside A,specnuezhenide,phillyrin,schisandrin,schisantherin A,deoxyschizandrin and schisandrin B) in Liuwei Wuling tablet (LWWLT).The HPLC baseline separation was carried out on an Inertsil(R) ODS-3 column (250 mm×4.6 mm,5 μm) at 25 ℃ with a gradient elution system composed of 0.1％ phosphoric acid solution and acetonitrile.The detection wavelength was set at 230 nm.Calibration curves for seven compounds showed good linear regressions,with correlation coefficients exceeding 0.999 within the tested concentration range.The limits of detection and quantification of each compound were in the range of 0.03-0.28 μg/mL and 0.11-0.87 μg/mL,respectively.The relative standard deviation values of precision,stability and repeatability were less than 2.34％.The average recovery of all seven constituents ranged from 96.71％ to 103.9％.The validated quantification method was successfully applied for the analysis of 10 batches of commercial LWWLT from Shandong Shibo Jindu Pharmaceutical Co.,Ltd.These results would make foundations for quality control and the further pharmacology study of LWWLT.