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目的 探讨肝癌细胞MGMT表达水平与奥沙利铂敏感性的相关性.方法 采用体外培养SMMC-7721、HuH-7和BEL-7402肝癌细胞株.用RT-PCR法检测MGMT的mRNA表达,Western-blot检测MGMT蛋白水平,MTT法检测奥沙利铂对肝癌细胞的抑制作用,并通过甲基化特异性PCR,检测所有细胞的MGMT甲基化的状态.结果 各肝癌细胞系均可检测到MGMT基因和蛋白的表达,其中HuH-7 MGMT的mRNA以及蛋白水平最高;而BEL-7402细胞MGMT的mRNA以及蛋白水平最低,对比MTT IC50显示奥沙利铂对HuH-7的敏感性最低,对BEL-7402敏感性最高.结论 MGMT基因状态可能影响肝癌细胞对奥沙利铂化疗的敏感性.“,”Objective To investigate the correlation between the expression of MGMT and the sensitivity of oxaliplatin in hepatocellular carcinoma cells. Methods SMMC-7721, HuH-7, BEL-7402 hepatocellular carcinoma cell lines were cultured in vitro. The expression of MGMT was detected by RT-PCR, the protein level of MGMT was detected by Western-blot, the inhibition of oxaliplatin on hepatocellular carcinoma cells was detected by MTT, and the methylation status of MGMT in all cells was detected by methylation-specific PCR. Results The expression of MGMT gene and protein could be detected in all hepatocellular carcinoma cell lines, and the level of HuH-7 MGMT was the highest, while that of BEL-7402 was the lowest. Compared with MTT IC50, oxaliplatin showed the lowest sensitivity to HuH-7 and the highest sensitivity to BEL-7402. Conclusion MGMT gene status may affect the sensitivity of hepatocellular carcinoma cells to oxaliplatin chemotherapy.