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为了探讨药物作用的时辰性,笔者按时辰药理学(Chronopharmacology)原理,考察了胰岛素降小白鼠血糖作用的昼夜节律变化。文献报道很多药物的昼夜节律,亦有大鼠等动物血糖及胰岛分泌胰岛素的节律研究,本文考察了外源性胰岛素降小白鼠血糖的昼夜节律。实验前先将雌性小白鼠置于21±1℃室温,12h光照—黑暗交迭的饲养室中驯养一周。然后在实验前8h禁食不停水,8h后用干实验。对照组(生理盐水0.04ml,SC)和实验组(胰岛素2U/kg.SC),在给药后15min断头取血清,用氧化酶法分别测正常血糖水平和胰岛素降血糖作用。
In order to investigate the timing of the effects of drugs, the authors investigated the circadian rhythm of blood glucose in insulin-deprived mice according to Chronopharmacology principles. The circadian rhythms of many drugs have been reported in the literature. There are also rhythm studies on blood glucose in rats and insulin secretion from islets. This article examined the circadian rhythm of exogenous insulin-lowering blood glucose in mice. Before the experiment, female mice were placed in a 21±1°C room temperature and domesticated for 12 weeks in a light-dark breeding room. Then the rats were fasted for 8 h before the experiment and dry experiments were performed after 8 h. In the control group (normal saline 0.04 ml, SC) and experimental group (insulin 2 U/kg. SC), serum was decapitated 15 minutes after administration, and the normal blood sugar level and insulin hypoglycemic effect were measured by oxidase method.