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目的观察罗格列酮对心肌梗死后大鼠心肌细胞凋亡过程中NF-КBP65表达的影响。方法45只雄性wistar大鼠随机分为假手术组(B组,n=15)和心肌梗死组(n=30),通过结扎左冠状动脉前降支(LAD)制作急性心肌梗死模型。模型制作成功24h后,心肌梗死组再随机分为心肌梗死对照组(A组,n=15)和罗格列酮干预组(C组,n=15)。罗格列酮干预组每日给以罗格列酮灌胃(4mg/kg),假手术组和心肌梗死对照组每日给以等量生理盐水灌胃,持续8周。利用免疫组化法检测非梗死区NF-КBP65(nuclear factor-КBP65)的含量。结果给药8周后,A组非梗死区心肌中NF-КBP65的表达,心肌细胞凋亡率,左心重量指数与B、C组相比显著升高,差异有统计学意义(P<0.05);C组与B组相比,非梗死区心肌中NF-КBP65的表达,心肌细胞凋亡率,左心室重量指数显著升高,差异有统计学意义(P<0.05)。结论罗格列酮可能通过抑制NF-КBP65的表达来改善大鼠心肌梗死后心肌细胞凋亡。
Objective To observe the effect of rosiglitazone on the expression of NF-КBP65 in rat cardiomyocyte apoptosis after myocardial infarction. Methods 45 male Wistar rats were randomly divided into sham operation group (n = 15 in group B) and myocardial infarction group (n = 30). Acute myocardial infarction model was made by ligating left anterior descending coronary artery (LAD). After the model was made successfully for 24 hours, the myocardial infarction group was randomly divided into myocardial infarction control group (group A, n = 15) and rosiglitazone intervention group (group C, n = 15). The rosiglitazone intervention group was given rosiglitazone orally daily (4mg / kg), and the sham operation group and the myocardial infarction control group were orally administered with the same amount of normal saline daily for 8 weeks. Immunohistochemistry was used to detect the content of NF-КBP65 in non-infarcted area. Results After 8 weeks of administration, the expression of NF-КBP65, myocardial cell apoptosis rate and left ventricular mass index in group A were significantly higher than those in group B and C (P <0.05) Compared with group B, the expression of NF-КBP65, myocardial cell apoptosis rate and left ventricular mass index in group C were significantly higher than those in group B (P <0.05). Conclusion Rosiglitazone may improve cardiomyocyte apoptosis after myocardial infarction in rats by inhibiting the expression of NF-КBP65.