论文部分内容阅读
目的:观察人参皂甙单体Rg3对肝癌新生血管及免疫功能的调节。方法:在体外培养的肝癌细胞,用凋亡检测筛选最佳作用浓度。24只C57BL/6小鼠随机分为4组,每组6只,皮下接种肝癌肿瘤细胞,除对照组以外,其它3组分别给予环磷酰胺(CTX组),人参皂甙Rg3(Rg3组),环磷酰胺联合人参皂甙Rg3(CTX+Rg3组),灌胃14d,检测肿瘤新生微血管密度计数(CD34)、细胞及体液免疫因子。结果:CTX+Rg3组肿瘤新生微血管密度计数(CD34)明显低于对照组(P<0.05);与CTX组比较,TNF-α和IL-10水平明显降低(P<0.01),CD4、CD8和CD4/CD8水平明显升高(P<0.01)。结论:人参皂甙单体Rg3通过抑制肿瘤新生微血管及调节免疫对小鼠肝癌移植瘤产生抑制作用.
Objective: To observe the regulation of ginsenoside Rg3 on neovascularization and immune function of hepatocellular carcinoma. Methods: The hepatoma cells cultured in vitro were screened for the optimal concentration by apoptosis assay. Twenty-four C57BL / 6 mice were randomly divided into 4 groups with 6 mice in each group. HCC tumor cells were inoculated subcutaneously, except CTX group, CTX group, Rg3 group, Cyclophosphamide combined with ginsenoside Rg3 (CTX + Rg3 group) was administered intragastrically for 14 days to detect the neoplastic microvessel density (CD34), cell and humoral immune factors. Results: The number of neovascular microvessel density (CD34) in CTX + Rg3 group was significantly lower than that in control group (P <0.05). Compared with CTX group, the levels of TNF-α and IL-10 were significantly decreased CD4 / CD8 levels were significantly higher (P <0.01). Conclusion: Ginsenoside Rg3 can inhibit the growth of transplanted hepatocellular carcinoma in mice by inhibiting the neovascularization and regulating immunity.