草鱼细胞和病毒的大规模培养研究

• 作 者: 曾令兵 高梦雪 蒋勇华
• 机 构: 中国水产科学研究院长江水产研究所
• 出 处: 淡水渔业
• 发表日期: 1995年06期
• 关键词: 草鱼出血病 细胞培养技术 病毒培养 细胞生长速度 静置培养 细胞增殖速度 病毒性鱼病 叶雪平 细胞培养物 长江水产研究所

本文对草鱼细胞和病毒的两种大规模培养方式进行了研究和比较。静置培养条件下，细胞生长速度快，２—３天内可形成单层，单层均匀而稳定；旋转培养条件下，细胞７天左右长成单层，细胞单层面积大，病毒培养产量高。这两种培养方式所观察到的细胞病变过程及表征基本相同，病毒的ＴＣＩＤ５０／ｍｌ值稳定在６．０左右。采用这两种培养方式，可实现草鱼细胞和病毒的大规模培养。

In this paper, two large-scale culture methods of grass carp cells and viruses were studied and compared. Under static culture conditions, the cell growth rate is fast, forming a single layer in 2-3 days, the single layer is uniform and stable; under the condition of rotating culture, the cells grow into a monolayer for about 7 days, the cell monolayer area is large, and the virus culture yield is high . The cell pathological changes and characterization observed by these two culture methods are basically the same, and the TCID50 / ml of the virus is stable at about 6.0. Using these two training methods, large-scale cultivation of grass carp cells and viruses can be achieved.