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本文报道了高压液相色谱分离,荧光检测,以三氯甲烷为溶剂直接进样测定植物油中α-生育酚含量的方法。采用Zorbax SIL色谱柱,流动相为正己烷:二噁烷:乙醇=97.6:2:0.4。对几种食用植物油中α-生育酚的测定结果表明,变异系数为5.7—7.9％。
This paper reports the determination of α-tocopherol in vegetable oils by high pressure liquid chromatography, fluorescence detection and direct injection with chloroform as solvent. A Zorbax SIL column was used with a mobile phase of n-hexane: dioxane: ethanol = 97.6: 2: 0.4. The results of the determination of α-tocopherol in several edible vegetable oils showed that the coefficient of variation was 5.7-7.9%.