Porous silicon optical cavity as an immunosensor platform

• 作 者: 吕小毅 莫家庆 涂亦娴 钟福如 江涛 贾振红 李江伟 张富春
• 机 构: School of Electronic and Information Engineering,Xi'an Jiaotong University,College of Information Sc
• 出 处: Optoelectronics Letters
• 发表日期: 2010年04期
• 关键词: polyclonal immunoassay label specificity monitored detecting porous immunosorben

A novel porous silicon based optical Fabry-Perot cavity structure is prepared as a label-free immunosensor platform for detecting antigen-antibody.The lagurus zona pelluciad 3(LZP3) and the specificity of the polyclonal anti-LZP3 antibodies are employed in our laboratory as the target and the probe,respectively.Firstly,the antibodies are immobilized to the porous silicon optical cavity using silanization and glutaraldehyde(GA) chemistry.And then,after the antigen-antibody reaction,it is monitored that the red shift of the reflection spectrum of the immunosensor increases with the antigen concentration.This research also plays a potential role for the extensive applications in immunoassay.

A novel porous silicon based optical Fabry-Perot cavity structure is prepared as a label-free immunosensor platform for detecting antigen-antibody. The lagurus zona pelluciad 3 (LZP3) and the specificity of the polyclonal anti-LZP3 antibodies are used in our laboratory as the target and the probe, respectively. Firstly, the antibodies are immobilized to the porous silicon optical cavity using silanization and glutaraldehyde (GA) chemistry. And then, after the antigen-antibody reaction, it is monitored that the red shift of the reflection spectrum of the immunosensor increases with the antigen concentration. this research also plays a potential role for the extensive applications in immunoassay.