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Objective To study the influence of salivary lipids on the adherence of cariogenic bacteria (S.mutans Ingbritt and BF; S.sobrinus OMZ176 and 6715). Methods Mixed saliva was collected from 10 healthy subjects (5 males and 5 females, aged 21～23). The salivary lipids was depleted under nondenatural condition (-10℃, ethanol∶ether 3∶2 V∶V ). The adherence of S.mutans and S.sobrinus to the experimental pellicles formed from mixed saliva or lipids-depleted saliva was investigated with 3H-TdR incorperation assay. Results The amount of S.mutans Ingbritt and BF adhered to lipids-depleted pellicle were significantly increased ( P <0.05). The amount of experimental bacteria adhered to lipids-depleted pellicle or mixed salivary pellicle were following the regular pattern: Ingbritt>BF>OMZ176>6715. Conclusion Salivary lipids significantly decreased the number of S.mutans adhered to pellicle.
Objective To study the influence of salivary lipids on the adherence of cariogenic bacteria (S. mutans Ingbritt and BF; S. sobrinus OMZ 176 and 6715). Methods Mixed saliva was collected from 10 healthy subjects (5 males and 5 females, aged 21-23 The adherence of S. mutans and S. sobrinus to the experimental pellicles formed from mixed saliva or lipids-depleted saliva was Results The amount of S. mutans Ingbritt and BF adhered to lipids-depleted pellicle were significantly increased (P <0.05). The amount of experimental bacteria adhered to lipids-depleted pellicle or mixed salivary pellicle were the regular pattern: Ingbritt> BF> OMZ176> 6715. Conclusion Salivary lipids significantly decreased the number of S. mutans adhered to pellicle.