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目的探讨良、恶性血液病以及肝病患者血清红细胞葡萄糖-6-磷酸脱氢酶(G-6-PD)、丙酮酸激酶(PK)和腺苷脱氨酶(ADA)酶活性的变化。方法收集148份红细胞酶检测标本,按病情分为良性血液病组(48例)、恶性血液病组(50例)、肝病组(20例)和健康对照组(30例)。采用罗氏全自动生化分析仪和罗氏原装试剂检测血清G-6-PD、PK和ADA水平。结果与健康对照组比较,良性血液病组、恶性血液病组和肝病组均不同程度存有红细胞酶的缺陷(P<0.05)。肝病组20例中,各型病毒性肝炎及肝炎后肝硬化患者的G-6-PD活性普遍降低,PK活性降低,ADA活性有升高,也有降低。14例发生黄疸,7例发生溶血。结论除良、恶性血液病患者存在红细胞酶缺陷外,肝炎后肝硬化患者也可以引起获得性红细胞酶缺陷,检测红细胞酶系变化可为临床某些疾病的诊断、鉴别诊断、治疗及免疫功能的研究提供参考。
Objective To investigate the changes of serum erythrocyte glucose-6-phosphate dehydrogenase (G-6-PD), pyruvate kinase (PK) and adenosine deaminase (ADA) activity in patients with benign and malignant hematological diseases and liver diseases. Methods 148 specimens of erythrocytes were collected and divided into benign hematological diseases (48 cases), hematological malignancies (50 cases), liver disease group (20 cases) and healthy control group (30 cases). Serum G-6-PD, PK and ADA levels were detected by Roche automatic biochemical analyzer and Roche original reagent. Results Compared with the healthy control group, the erythrocyte enzyme deficiency was found in benign blood disease group, hematologic malignant disease group and liver disease group (P <0.05). In 20 cases of liver disease group, the activity of G-6-PD in all kinds of viral hepatitis and posthepatitis cirrhosis patients were generally lower, PK activity decreased, ADA activity increased, but also decreased. 14 cases of jaundice, hemolysis occurred in 7 cases. Conclusion In addition to the presence of erythrocyte enzyme deficiency in patients with benign and malignant hematological diseases, patients with posthepatitic cirrhosis can also cause acquired erythrocyte enzyme deficiency, and detection of changes in erythrocyte enzymes may be the diagnosis, differential diagnosis, treatment and immune function of certain diseases in clinical practice Research for reference.