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目的:使用基因芯片技术研究重症肌无力(MG)患者外周血单个核细胞免疫相关基因的差异表达。方法:采用BIOSTAR Human-6-V3型人类全长基因cDNA表达谱芯片筛选30例初次发病MG患者差异表达的免疫相关基因,酶联免疫吸附法(ELISA)检测患者外周血IL-6、TNF-α的水平,对MG患者病情按许贤豪绝对评分法进行评分,并比较其临床意义。结果:158条差异表达免疫相关基因中,表达上调的基因76条(Ratio值2.0倍以上),表达下调的基因82条(Ratio值0.5倍以下),差异表达的免疫相关基因主要涉及细胞因子及其受体、趋化因子及其受体、细胞黏附分子、白细胞分化抗原、免疫转录调节分子、天然免疫分子等,细胞因子中IL-6、TNF-αmRNA表达水平显著上调,与MG患者血清IL-6、TNF-α水平检测结果一致,血清IL-6、TNF-α水平与MG临床绝对评分呈正相关。结论:人类全基因芯片技术可以快速、高通量筛选出MG患者差异表达的免疫相关基因,结果证实多个免疫基因参与了MG发病过程,为进一步阐明MG发病的免疫机制及治疗提供新的思路。
OBJECTIVE: To study the differential expression of immune-related genes in peripheral blood mononuclear cells in patients with myasthenia gravis (MG) using gene chip technique. Methods: Thirty cases of differentially expressed immune-related genes in primary MG patients were screened by BIOSTAR Human-6-V3 full-length human cDNA microarray. Enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of IL-6 and TNF- α levels, the severity of MG patients by Xu Xianhao absolute score score, and compare its clinical significance. Results: Of the 158 differentially expressed immune-related genes, 76 genes were up-regulated (2.0 times above) and 82 genes were down-regulated (Ratio value was 0.5 times or less). The differentially expressed immune related genes mainly involved cytokines and Its receptors, chemokines and their receptors, cell adhesion molecules, leukocyte differentiation antigen, immune transcription regulatory molecules, natural immune molecules, cytokines IL-6, TNF-αmRNA expression levels were significantly increased, and MG serum IL -6, and TNF-α levels. Serum levels of IL-6 and TNF-α were positively correlated with clinical absolute scores of MG. Conclusion: The human whole-gene microarray technique can screen the immune-related genes differentially expressed in MG patients by rapid and high-throughput. The results show that multiple immune genes are involved in the pathogenesis of MG, providing new ideas for further elucidating the immune mechanism and treatment of MG .