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目的探讨硒、锌联合作用对氟致小鼠成釉细胞DNA损伤的影响。方法取体外培养的处于对数生长期的小鼠成釉细胞,随机设立对照组(10%DMEM培养基)、(0.25、0.50、1.00、2.00、4.00 mmol/L氟化钠)氟单独作用组和低剂量硒+锌联合组(2.50μmol/L亚硒酸钠+10.00μmol/L硫酸锌)、高剂量硒+锌联合组(5.00μmol/L亚硒酸钠+20.00μmol/L硫酸锌)及低剂量硒+锌+氟联合组(2.50μmol/L亚硒酸钠+10.00μmol/L硫酸锌+0.25、0.50、1.00、2.00、4.00 mmol/L氟化钠)、高剂量硒+锌+氟联合组(5.00μmol/L亚硒酸钠+20.00μmol/L硫酸锌+0.25、0.50、1.00、2.00、4.00 mmol/L氟化钠),硒+锌+氟联合组先经亚硒酸钠和硫酸锌预处理24 h后,再分别给予氟化钠染毒。培养24 h后,采用单细胞凝胶电泳技术(SCGE)检测DNA损伤情况。结果与对照组比较,氟单独作用组小鼠成釉细胞的尾长、Olive尾矩、尾DNA%和尾长/头长值均升高(P<0.05)。与相同剂量氟单独作用组比较,低、高剂量硒+锌+各剂量氟联合组小鼠成釉细胞的尾长、Olive尾矩和尾长/头长值降低(P<0.05)。与低剂量硒+锌+氟联合组比较,高剂量硒+锌+各氟联合组小鼠成釉细胞的Olive尾矩总体升高(P<0.05)。结论过量摄入氟化钠可致小鼠成釉细胞DNA损伤,而硒、锌联合作用对氟致小鼠成釉细胞DNA损伤有一定的拮抗作用,且低剂量硒、锌联合作用的拮抗效果更为明显。
Aim To investigate the effect of selenium and zinc on DNA damage in amelogenin-induced murine ameloblasts. Methods Mouse ameloblasts cultured in vitro were randomly divided into four groups: control group (10% DMEM), (0.25,0.50,1.00,2.00,4.00 mmol / L sodium fluoride) fluoride alone group (2.50μmol / L sodium selenite + 10.00μmol / L zinc sulfate), high dose selenium + zinc combined group (5.00μmol / L sodium selenite + 20.00μmol / L zinc sulfate) (2.50μmol / L sodium selenite + 10.00μmol / L zinc sulfate + 0.25, 0.50, 1.00, 2.00, 4.00mmol / L sodium fluoride), high dose of selenium + zinc + Fluoro combination group (5.00μmol / L sodium selenite + 20.00μmol / L zinc sulfate + 0.25, 0.50,1.00,2.00,4.00 mmol / L sodium fluoride), selenium + zinc + And zinc sulfate pretreatment 24 h, then were given sodium fluoride exposure. After cultured for 24 h, the DNA damage was detected by single cell gel electrophoresis (SCGE). Results Compared with the control group, tail length, Olive tail moment, tail DNA% and tail length / head length of ameloblasts in fluoride-treated mice were significantly increased (P <0.05). Compared with the same dose of fluoride alone group, the tail length, Olive tail moment and tail length / head length of ameloblasts in low and high doses of selenium + zinc combined with each dose of fluorine combined group decreased (P <0.05). Compared with the low-dose selenium + zinc + fluoride group, Olive tail moment of ameloblasts in high-dose selenium + zinc combined with each fluoride group was generally increased (P <0.05). Conclusions Excessive ingestion of sodium fluoride can cause DNA damage in ameloblasts of mice, while the combined effects of selenium and zinc have some effects on the DNA damage induced by fluoride-induced ameloblasts, and the antagonistic effects of low-dose selenium and zinc combined action More obvious.