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Voltage-dependent and Ca~(2+)-activated K~+ channels of the primary cultured coronary artery smooth muscle cells from porcine heart were investigated using the patch-clamp technique. The results are summarized as follows:1). In the inside-out configuration, under quasi-physiological cation gradients conditions(normal Tyrode’s solution outside. K~+-rich inside), at a membrane potential of-90mV no discernible channel currents were recorded. Depolarizing the membrane patch revealed a outward single-channel current, and the pattern of the channel activity appeared to be relative to changes in the voltage applied to the membrane. When membrane potentials became more positive, the rectangular-shaped currents with a large amplitude
Voltage-dependent and Ca ~ (2 +) - activated K ~ + channels of the primary cultured coronary artery smooth muscle cells from porcine heart were investigated using the patch-clamp technique. The results are summarized as: 1). In the inside -out configuration, under quasi-physiological cation gradients conditions (normal Tyrode’s solution outside. K ~ + -rich inside), at a membrane potential of -90mV no discernible channel currents were recorded. Depolarizing the membrane patch revealed a outward single-channel current , and the pattern of the channel activity appeared to be relative to changes in the voltage applied to the membrane. When membrane potentials became more positive, the rectangular-shaped currents with a large amplitude