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目的了解河南省吸毒人群HIV-1的亚型分布及流行特征。方法采集27例HIV-1抗体阳性的吸毒者外周血单个核细胞(PBMC),应用套式聚合酶链反应(PCR)对其核酸样品进行扩增并测序,对其env基因C2-V3段及邻近350～450个核酸序列进行比较分析。结果27份血样中,6份为BC重组亚型,其中4份为CRF07-BC,2份为CRF08-BC。与国际参考株CRF07-BC.CN.97.CN54A.CRF08-BC.97CNGX6F.接近。21份为泰国B(B’)亚型,与国际参考株RL42接近。结论河南省吸毒人群感染的HIV-1以在中国中部地区献血人群中流行的泰国B(B′)亚型为主,也存在主要以性传播途径感染的人群中流行的BC重组亚型。
Objective To understand the subtypes and epidemic characteristics of HIV-1 among drug users in Henan Province. Methods Peripheral blood mononuclear cells (PBMCs) were collected from 27 HIV-1 positive drug users. The nucleic acid samples were amplified and sequenced by nested polymerase chain reaction (PCR) Near 350 ~ 450 nucleic acid sequences for comparative analysis. Results Of 27 blood samples, 6 were BC recombinant subtypes, of which 4 were CRF07-BC and 2 were CRF08-BC. Close to international reference strain CRF07-BC.CN.97.CN54A.CRF08-BC.97CNGX6F. 21 were Thai B (B ’) subtypes, which was close to the international reference strain RL42. Conclusions HIV-1 infected with drug addicts in Henan Province is predominantly in Thailand B (B ’) subtype prevailing in blood donors in central China. There are also BC subtypes that are prevalent among those infected mainly by sexually transmitted infections.