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采用原子力显微镜(AFM)对改性前后材料表面粘附生长的角膜基质细胞的亲和力、三维形貌和力学性能进行了分析。结果显示,改性后材料的细胞亲和力有较大改善,且材料表面细胞三维形态更加正常,铺展更为舒展,与材料的接触面积较大。细胞力学性能分析也发现改性后材料表面细胞具有更高的粘附力和杨氏模量,以及更低的硬度,说明未改性p(HEMA-MMA)材料具有明显的细胞毒性,这种毒性作用导致在其表面生长的细胞的细胞骨架遭到破坏,细胞健康状态明显不如改性后材料表面细胞。因此,改性后的Col/bFGF-p(HEMA-MMA)更适合作为人工角膜材料使用。
Atomic force microscopy (AFM) was used to analyze the affinity, three-dimensional topography and mechanical properties of corneal stromal cells adhering to the surface of the material before and after modification. The results showed that the affinity of the modified material greatly improved, and the three-dimensional morphology of the material surface cells more normal spread more stretch, and the larger the contact area with the material. The analysis of the mechanical properties of the cells also showed that the modified cells had higher adhesion and Young’s modulus and lower hardness, indicating that the unmodified p (HEMA-MMA) material has obvious cytotoxicity. Toxicity results in destruction of the cytoskeleton of the cells growing on its surface, and the cell health status is significantly lower than that of the modified material surface cells. Therefore, the modified Col / bFGF-p (HEMA-MMA) is more suitable for use as an artificial corneal material.