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目的:探讨葡萄糖-6-磷酸异构酶(GPI)检测、及与抗环瓜氨酸多肽(CCP)抗体、类风湿因子(RF)联合检测在RA诊断中的价值。方法:用ELISA双抗体夹心法检测RA组、OA组和正常对照组GPI、抗CCP抗体和RF水平。结果:RA组GPI(1.9±1.3)mg/L、OA组(0.22±0.08)mg/L(P<0.01)、正常对照组(0.14±0.07)mg/L(P<0.01);GPI在RA活动组和非活动组差异有统计学意义(P<0.01);同时其对RA的灵敏度、特异度均高于抗CCP抗体、RF。经pearson检验,GPI与RA病人DAS28评分正相关(r=0.456,P<0.01)。结论:血清GPI对诊断RA、尤其是活动期RA有相对较高灵敏度和特异度、可作为RA诊断和评估的实验室指标。
Objective: To investigate the value of detection of glucose-6-phosphate isomerase (GPI), combined with anti-cyclic citrullinated peptide (CCP) antibody and rheumatoid factor (RF) in the diagnosis of RA. Methods: GPI, anti-CCP antibody and RF level in RA group, OA group and normal control group were detected by ELISA double antibody sandwich method. Results: The GPI in RA group was (1.9 ± 1.3) mg / L, in OA group was 0.22 ± 0.08 mg / L (P <0.01), and in normal control group was (0.14 ± 0.07) mg / The difference between active group and inactive group was statistically significant (P <0.01). Meanwhile, the sensitivity and specificity of RA to RA were higher than those of anti-CCP and RF. Pearson test showed that GPI was positively correlated with DAS28 score in RA patients (r = 0.456, P <0.01). Conclusion: Serum GPI has relatively high sensitivity and specificity for the diagnosis of RA, especially active RA, which can be used as a laboratory index for the diagnosis and assessment of RA.