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目的:通过动物实验,研究裸鼠信号转导及转录活化蛋白5(signal transducer and activator of transcription5,STAT5)诱杀寡脱氧核苷酸(decoy oligodeoxynucleotide,Decoy ODN)的抑瘤作用。方法:采用皮下注射的方法建立K562细胞裸鼠移植瘤模型,瘤体内注射脂质体-ODN混合物,观察裸鼠生长状况、瘤体大小等。分别采用反转录-聚合酶链反应(reverse tran-scription-polymerase chain reaction,RT-PCR)和Western印迹法检测瘤组织中bcl-xL、cyclin D1和c-myc的mRNA和蛋白表达水平的改变情况。结果:经Decoy ODN处理抑制了K562细胞对裸鼠的致瘤能力,瘤体积和质量明显小于突变ODN组[MutantODN组瘤重(0.93±0.22)gvsDecoy ODN组瘤重(0.485±0.178)g,P<0.05],肿瘤生长抑制率达47.7%。RT-PCR、Western印迹检测结果显示bcl-xL、cyclin D1和c-myc的mRNA和蛋白表达水平下调。结论:转录因子诱杀策略可以有效地在移植瘤裸鼠模型体内阻断STAT5靶基因的表达。
OBJECTIVE: To study the antitumor effect of Decoy ODN induced by signal transducers and activator of transcription5 (STAT5) in nude mice. Methods: The nude mouse xenograft models of K562 cells were established by subcutaneous injection. The liposome-ODN mixture was injected into the tumor in nude mice to observe the growth status and tumor size. The changes of mRNA and protein expression of bcl-xL, cyclin D1 and c-myc in tumor tissue were detected by RT-PCR and Western blot respectively Happening. Results: Decoy ODN treatment inhibited the tumorigenicity of K562 cells in nude mice. The tumor volume and quality were significantly lower than those in mutant ODN group [(0.93 ± 0.22) gvsDecoy ODN tumor weight (0.485 ± 0.178) g, P <0.05], tumor growth inhibition rate of 47.7%. The results of RT-PCR and Western blot showed that the mRNA and protein expressions of bcl-xL, cyclin D1 and c-myc were down-regulated. Conclusion: Transcription factor trapping strategy can effectively block the expression of STAT5 target gene in nude mice model.