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目的研究高浓度葡萄糖在斑马鱼胚胎早期发育中的作用以及对斑马鱼多巴胺神经元发育的影响。方法实验分为3组:空白对照组,葡萄糖实验(25 mmol/L葡萄糖)组,甘露醇对照(25 mmol/L甘露醇)组。25 mmol/L葡萄糖溶液处理Vmat-GFP转基因斑马鱼胚胎,当胚胎发育到第3天时,激光共聚焦显微镜观察多巴胺神经元的发育,荧光定量PCR检测PD相关基因的表达。结果与空白对照组、甘露醇组相比,25 mmol/L葡萄糖溶液处理胚胎后,胚胎发育迟缓,心脏畸形,多巴胺神经元荧光增强,端脑部位多巴胺神经元数量增加,PD(帕金森病)相关基因表达水平升高,其中parkin基因表达差异具有统计学意义(P<0.05)。结论 25 mmol/L葡萄糖溶液对胚胎的发育具有毒性作用,但在胚胎发育的早期对多巴胺神经元具有营养作用。
Objective To investigate the role of high glucose concentration in the early development of zebrafish embryos and its effect on the development of zebrafish dopamine neurons. Methods The experiment was divided into three groups: blank control group, glucose group (25 mmol / L glucose), mannitol group (25 mmol / L mannitol group). Vmat-GFP transgenic zebrafish embryos were treated with 25 mmol / L glucose solution. When the embryo developed to day 3, the development of dopaminergic neurons was observed by confocal microscopy. The expression of PD-related genes was detected by quantitative PCR. Results Compared with blank control group and mannitol group, embryonic development was delayed, fetal heart malformations and dopaminergic neurons were increased after treatment with 25 mmol / L glucose solution, and the number of dopamine neurons in the diencephalon increased. PD (Parkinson’s disease ), And the difference of parkin gene expression was statistically significant (P <0.05). Conclusion 25 mmol / L glucose solution has toxic effect on the development of embryos, but it has nourishing effect on dopamine neurons in the early stage of embryo development.