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目的:探讨NaCl胁迫对甘草生长、生理及有效成分含量的影响。方法:以药用植物甘草为材料,采用水培方式培养,以含有不同质量浓度NaCl的营养液对其进行不同强度的盐胁迫处理,分别于胁迫后10,20,30,40 d动态取样,测定甘草的生长、生理指标和甘草酸、甘草苷含量。结果:处理20,30,40 d时,0.6%和0.8%处理组的甘草植株株高、甘草根鲜重显著低于对照组;处理30,40 d时,0.6%和0.8%处理组的甘草干重均显著低于对照组;处理20 d,0.6%和0.8%处理组的甘草叶片SOD酶活性和叶绿体色素的含量均极显著高于对照组;处理30 d,0.6%和0.8%NaCl处理的甘草中甘草酸极显著高于对照组,处理20 d和30 d,0.6%,0.8%NaCl处理的甘草苷含量极显著高于对照组。结论:NaCl胁迫下,甘草生长受到了抑制,并通过提高体内的抗氧化酶活性、降低叶绿体色素含量和促进次生代谢产物积累,来提高自身耐盐性,同时也提高了药材的质量。
Objective: To investigate the effects of NaCl stress on the growth, physiological and active ingredients of Glycyrrhiza uralensis. Methods: The medicinal plant licorice was used as the material and cultured by hydroponic culture. The nutrient solution containing different concentrations of NaCl was used to treat it with different intensities of salt stress. Dynamic samples were taken at 10, 20, 30 and 40 d after stress, Determination of licorice growth, physiological indicators and glycyrrhizin, glycyrrhizin content. Results: At 20, 30 and 40 days, the fresh weight of licorice root and fresh weight of licorice root in 0.6% and 0.8% treatment groups were significantly lower than those in control group. When treated with 0.6% and 0.8% Dry weight were significantly lower than that of the control group; SOD activity and chloroplast pigment content of licorice leaves were significantly higher than those of the control group at 20 d, 0.6% and 0.8% treatment; 30 d, 0.6% and 0.8% NaCl treatment Of glycyrrhizic acid glycyrrhizic acid was significantly higher than the control group, the treatment of 20 d and 30 d, 0.6%, 0.8% NaCl glycyrrhizin content was significantly higher than the control group. CONCLUSION: The growth of Glycyrrhiza uralensis was inhibited under NaCl stress and its salt tolerance was improved by increasing the antioxidant enzyme activity, decreasing the content of chloroplast pigment and promoting the accumulation of secondary metabolites in the body. Meanwhile, the quality of medicinal materials was also improved.