论文部分内容阅读
人乳头瘤病毒(Human Papilomavirus,HPV)是一种具有种属特异性的嗜上皮类病毒,为闭环双链的小 DNA 病毒,包含约8000 bp,无包膜,分类学上属乳头瘤病毒科(Papovaviridae)下的乳头瘤病毒属.其基因组包含8个早期开放读码框架(E:1-8)、2个晚期读码框架(L:1-2)及1个非编码调控区.晚期读码框 L1和 L2基因分别编码 HPV 的主要和次要衣壳蛋白,组装成 HPV 的衣壳.L1是衣壳的主要蛋白,分子量约57kDa.L1是所有乳头状瘤病毒最为保守的基因, L1蛋白可诱导人的机体产生中和反应,并起免疫保护作用,具有 B 细胞特异抗原表位[1].乳头瘤病毒具有高度的宿主特异性,人类是 HPV 的单纯宿主,主要感染受损的皮肤和黏膜,病毒在感染组织中的滴度较低,目前尚未建成有效的 HPV 体外培养技术[2].“,”Human papiloma virus ( Human Papilomavirus, HPV ) is a kind of is genus specific tropism for epithelial virus, for closed loop and double chain of smal DNA virus, contains about 8000 BP, no capsule, taxonomy of papilomavirus ( Papovaviridae ) of papilomavirus. The genome contains 8 early open reading frame ( E:1-8 ), 2 advanced reading frame ( L:1-2) and 1 non-coding regulatory region. Advanced reading frame of L1 and L2 gene were encoded in HPV major and minor capsid protein, assembled into HPV capsid. L1 is the major capsid protein, molecular weight of about 57kDa. L1 is al the papiloma virus is the most conserved gene, L1 protein can be induced in the human body to produce the neutralization reaction, and immune protective effect, with B cels specific for epitopes of [1]. Papiloma virus has a high degree of host specificity, human is the HPV simple host, primary infection of damaged skin and mucosa, virus infection in tissues of titre was low, at present has not yet been built effective HPV in vitro technology [2].