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机体生命活动能量的主要来源是生物氧化,当机体受到某些外界因素、药物等影响或处于不同生理、病理和药理状态时,氧摄取量和氧耗速率可发生改变。因此,从整体、组织、细胞器到酶分子反应有关氧耗的研究一直是生物学和医学实验研究的重要课题。Warburg测压技术测得的氧耗量是一定实验时间内生物体系(例如组织匀浆和片切)所摄取氧量的平均值,不能观察呼吸时氧耗的动态变化,亦不能进行自动测定。连续研究生物体系氧耗反应是呼吸动力学和生物能力学研究的基础,自Clark复膜电极出现后,
The main source of biological energy is biological oxidation. When the body is affected by some external factors, drugs, or in different physiological, pathological and pharmacological states, oxygen uptake and oxygen consumption rate may change. Therefore, the study of oxygen consumption from the whole body, tissues, organelles to the reaction of enzyme molecules has been an important topic in biological and medical experimental research. The oxygen consumption measured by Warburg is the average of the amount of oxygen taken by biological systems (such as tissue homogenates and slices) over a certain experimental period. The oxygen consumption can not be observed during respiration or automatically measured. Continuous oxygen consumption in biological systems is the basis of respiratory dynamics and bioequivalence studies. Since the advent of Clark Fumo electrodes,