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目的对比研究基因芯片与液体快速培养技术诊断耐药结核病的效果。方法 1156例疑似肺结核及肺结核患者的同一痰样本,同时应用基因芯片技术(芯片组)与液体快速培养技术(快培组)进行结核菌耐药检测,对两组检测结果进行对比分析。结果对两组实验检测结果进行对比分析,结核菌检出率:芯片组270例,阳性率为23.36%,快培组237例,阳性率为20.50%。结核菌耐药结果 :芯片组46例,耐药率为17.04%,快培组43例,耐药率为18.14%,比较差异无统计学意义(P>0.05)。芯片组与快培组同时检出结核菌阳性时,二者耐药符合率达80%。结论基因芯片检测结合聚合酶链式反应(PCR)技术和反向杂交技术(RDB)诊断结核病及耐药结核病,具有简便、快速、灵敏、准确的特点,及时检出结核病及耐药结核病,指导临床针对性制定个性化抗结核方案,避免盲目制定抗结核方案而导致医源性耐药结核病的出现,真正有效控制结核病及耐药结核病。
Objective To compare the effects of DNA microarray and liquid rapid culture to diagnose drug-resistant tuberculosis. Methods A total of 1,156 cases of suspected sputum from patients with pulmonary tuberculosis and tuberculosis were tested for tuberculosis drug resistance by gene chip technology (chipset) and liquid rapid culture technology (fast training group), and the results of the two groups were compared. Results The results of two groups of tests were compared and analyzed. The detection rate of TB was 270 cases in chipset group, the positive rate was 23.36%, and 237 cases in fasting group, the positive rate was 20.50%. Mycobacterium tuberculosis drug resistance results: chipset 46 cases, the resistance rate was 17.04%, fasting group 43 cases, the drug resistance rate was 18.14%, the difference was not statistically significant (P> 0.05). Chips group and fast culture group also detected positive TB, the two resistance coincidence rate of 80%. Conclusion Gene chip detection combined with polymerase chain reaction (PCR) and reverse hybridization (RDB) in the diagnosis of tuberculosis and drug-resistant tuberculosis is simple, rapid, sensitive and accurate, and timely detection of tuberculosis and drug-resistant tuberculosis, guidance Targeted clinical development of personalized anti-TB program to avoid the blind development of anti-TB program led to the emergence of iatrogenic drug-resistant tuberculosis, the real effective control of tuberculosis and drug-resistant TB.