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目的研究拉坦前列素(latanoprost,LAT)脂质体滴眼液对眼表的毒性。方法采用薄膜分散法制备拉坦前列素脂质体,平均粒径为(98.7±34.4)nm;采用随机数字表法将新西兰白兔15只分为3组,分别为Xalatan组、LAT Liposome组和无抑菌剂组,右眼给予试验滴眼液,左眼采用无菌生理盐水作为对照,1次/d给药,连续使用28 d;在每日给药前以及最后一次给药后的1、2、4、24、48、72 h进行眼刺激反应和角膜荧光素染色评分。结果 LAT Liposome组与无抑菌剂组在实验第28天刺激反应平均分值分别为0.96和2.24,属于无刺激,而Xalatan组评分值为4.17,属于轻度刺激;角膜荧光素染色实验中,给药后第21天,与对照组比较,Xalatan组评分开始显著升高(P<0.01);给药后第28天,与对照组比较,Xalatan组、无抑菌剂组评分均显著增加(P<0.01);与Xalatan组比较,LAT Liposome组评分显著降低(P<0.01),说明LAT Liposome对眼表刺激性最小,角膜损伤最轻。结论 LAT Liposome的毒性最小,更有利于长期使用。
Objective To study the ocular toxicity of latanoprost (LAT) liposome eye drops. Methods Rat lipopolysaccharide liposomes were prepared by thin film dispersion method and the mean diameter was (98.7 ± 34.4) nm. Fifteen New Zealand white rabbits were divided into three groups by random number table: Xalatan group, LAT Liposome group And no antibacterial agent group, the right eye to give test eye drops, the left eye using sterile saline as a control, 1 / d administration, continuous use 28 d; before daily administration and after the last administration The eye irritation and corneal fluorescein staining scores were evaluated at 1, 2, 4, 24, 48 and 72 h. Results The average response scores of LAT Liposome group and non-bacteriostatic group at the 28th day of experiment were 0.96 and 2.24, respectively, which were non-stimulated, while the score of Xalatan group was 4.17, which was mild stimulation. In corneal fluorescein staining , On the 21st day after administration, the score of Xalatan group began to increase significantly compared with the control group (P <0.01). On the 28th day after administration, the scores of Xalatan group and non-bacteriostatic agent group (P <0.01). Compared with Xalatan group, the score of LAT Liposome group was significantly lower (P <0.01), which indicated that LAT Liposome had the least eye irritation and the lightest corneal injury. Conclusion LAT Liposome has the least toxicity and is more conducive to long-term use.