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目的:应用基因芯片技术研究异紫堇碱处理后的Si Ha细胞差异基因表达谱改变,以寻找异紫堇碱抑制宫颈癌Si Ha细胞HPV基因表达的分子机理。方法:抽提经异紫堇碱处理后不同时段的Si Ha细胞和未经异紫堇碱处理的Si Ha细胞中的mRNA来制备探针,经杂交、洗涤后,通过计算机扫描分析未经异紫堇碱处理的Si Ha细胞和处理后的Si Ha细胞基因表达谱的差异情况。结果:在每组60000多个候选基因中,各筛选出200多条差异表达倍数在2倍以上的基因,在异紫堇碱处理后12小时的样本中下调基因206条,上调基因70条,未知基因217条;24小时后下调基因95条,上调基因137条,未知基因166条;48小时后下调基因150条,上调基因101条,未知基因159条。结论:基于基因芯片技术的基因表达谱分析能够高通量筛选与异紫堇碱抑制宫颈癌Si Ha细胞HPV基因表达的分子机理相关的基因。
OBJECTIVE: To study the molecular gene expression profile of Si Ha cells treated with corydaline by gene chip technique in order to find out the molecular mechanisms by which corydaline inhibits HPV gene expression in Si Ha cells. Methods: The probe was prepared by extracting the mRNA from Si Ha cells at different periods after treatment with corydaline and from Si Ha cells not treated with corydaline. After hybridization and washing, Differences in Gene Expression Profiles between Corydalis-treated Si Ha Cells and Treated Si Ha Cells. Results: Of the more than 60,000 candidate genes in each group, more than 200 genes were screened at more than two times of the differential expression level, 206 genes were upregulated in 12 hours after corynebacterium treatment, 70 genes were up-regulated, There are 217 unknown genes; 95 genes are down-regulated after 24 hours, 137 genes are up-regulated and 166 genes are unknown; 150 genes are down-regulated after 48 hours, 101 genes are up-regulated and 159 genes are unknown. Conclusion: Gene expression profiling based on gene chip technology can screen high-throughput genes related to the molecular mechanisms by which corydaline inhibits HPV gene expression in Si ha cells of cervical cancer.