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Objective To investigate effects of clenbuterol(CLB) on the peroxisome proliferatorsactivated receptor γ(PPARγ) expression in adipose tissues of rats.Methods Twenty adult female Sprague-Dawley rats were randomly divided into4 groups(5 rats per group). CLB solved in normal saline solution was given at the dose of 0 mg/kg body weight(bw)(group A, as the control), 0.4 mg/kg bw(group B,low-dose group), 2.0 mg/kg bw(group C, mid-dose group), and 18.5 mg/kg bw(group D,high-dose group) for 14 d by gavage consecutively, respectively. Methods of immunohistochemistry, quantitative Real-time PCR and Western blotting were performed to detect expression of PPARγ in the adipose tissue samples.Results PPARγ-positive immunostaining was strong in the controls and weak in the experimental groups. There was no difference on PPARγ mR NA and protein between the low-dose group and the control(P>0.05). With the increase of CLB doses, expression levels of PPARγ mR NA and protein were significantly lower in mid- or high-dose group than those in the control(P<0.01).Conclusions The PPARγ expression in adipose tissues of rats could be down-regulated after CLB exposure, and the decrease became more severe with the increasing doses.
Objective To investigate the effects of clenbuterol (CLB) on the peroxisome proliferators activated receptor γ (PPARγ) expression in adipose tissues of rats. Methods Twenty adult female Sprague-Dawley rats were divided into four groups (5 rats per group). CLB solved in normal saline solution was given at the dose of 0 mg / kg body weight (bw) (group A, as the control), 0.4 mg / kg bw (group B, low-dose group), 2.0 mg / kg bw -dose group, and 18.5 mg / kg bw (group D, high-dose group) for 14 d by gavage consecutively, respectively. Methods of immunohistochemistry, quantitative Real-time PCR and Western blot were performed to detect expression of PPARγ in the adipose tissue samples. Results PPARγ-positive immunostaining was strong in the controls and weak in the experimental groups. There was no difference on PPARγ mR NA and protein between the low-dose group and the control (P> 0.05). With the increase of CLB doses, expression levels of PPARγ mR NA and proteins were significantly lower in mid- or high-dose group than those in the control (P <0.01) .Conclusions The PPARγ expression in adipose tissues of rats could be down-regulated after CLB exposure, and the decrease became more severe with the increasing doses.