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目的 :探索食管癌细胞株 EC/ CU HK1、EC/ CU HK1(As- )对三氧化二砷 (As2 O3 )促凋亡作用敏感性与细胞活性氧 (reactive oxygen species,ROS)水平的关系。方法 :以二甲萘醌(DMNQ)孵育 EC/ CU HK1、EC/ CU HK1(As- )细胞 ,双氢 -乙酰乙酸二氯荧光黄 (2 ,7- dichlorodihy-drofluorescein diacetate,DCFH- DA)及双氢罗丹明 12 3(dihydrorhodamine12 3,DHR)捕获 ROS,流式细胞仪检测两种细胞 ROS水平的差异。 As2 O3 单独或联用 DMNQ孵育 EC/ CU HK1、EC/ CUHK1(As- )细胞 ,流式细胞仪 Tunel法分析两种细胞凋亡敏感性的差异及其在用药前后的变化。结果 :EC/CUHK1的 ROS水平明显高于 EC/ CUHK1(As- ) ,DMNQ可提高 EC/ CU HK1、EC/ CU HK1(As- )的ROS水平 ,诱发 EC/ CUHK1(As- )对 As2 O3 的敏感性 ,增强 As2 O3 促 EC/ CU HK1(As- )细胞凋亡的效应。过氧化氢酶 (catalase)可逆转 DMNQ的效应。结论 :食管癌细胞株 EC/ CUHK1、EC/ CUHK1(As- )对 As2 O3 促凋亡的敏感性决定于细胞固有的 ROS水平
Objective: To investigate the relationship between the sensitivity of esophageal cancer cell lines EC / CU HK1, EC / CU HK1 (As-) to the apoptosis of As2 O3 cells and the level of reactive oxygen species (ROS). Methods: EC / CU HK1, EC / CU HK1 (As-) cells were incubated with 2, 7-dichlorodihy-drofluorescein diacetate (DCFH- DA) ROS was detected by dihydrorhodamine 12 3 (DHR), and the differences of ROS levels between the two cells were detected by flow cytometry. As2 O3 alone or in combination with DMNQ incubated EC / CU HK1, EC / CUHK1 (As-) cells, flow cytometry Tunel analysis of the difference between the two apoptosis and its changes in the medication before and after. Results: The ROS level of EC / CUHK1 was significantly higher than that of EC / CUHK1 (As-). DMNQ increased ROS levels of EC / CU HK1 and EC / CU HK1 (As-) , And enhance the effect of As2 O3 on the apoptosis of EC / CU HK1 (As-) cells. Catalase reverses the effects of DMNQ. Conclusion: The sensitivity of esophageal cancer cell lines EC / CUHK1 and EC / CUHK1 (As-) to the apoptosis of As2O3 is determined by the intrinsic ROS level