Effects of different doses of acitretin on FGF10 mRNA transcription and its protein translation in H

• 作 者: YU Chun-shui TAN Sheng-shun SUI Wei-chi XI Yan-ping
• 机 构: Department of Dermatology, Second Affiliated Hospital, Medical School of Xi’an Jiaotong University,X
• 出 处: 中国人民解放军军医大学学报（英文版）
• 发表日期: 2006年6期
• 关键词: acitretin HaCaT cells FGF10 immunofluorescence in situ hybridization

Objective:To observe the effects of different doses of acitretin on the transcription of FGF10 mRNA and the translation of FGF10 protein in cultured HaCaT cells. Methods: HaCaT cells were treated with different doses of acitretin for 48 h, then the changes on the transcription of FGF10 mRNA and the translation of FGF10 protein in these cells were detected by immunofluorescence and in situ hybridization assay. Results: Compared with the control group, the transcription of FGF10 mRNA and the translation of FGF10 protein were gradually decreased along with the increasing dose of acitretin. There were significant differences between different groups (P＜0.01). Conclusion: Acitretin could inhibit the transcription of FGF10 mRNA and the translation of FGF10 protein in HaCaT cells. With the dose of acitretin increased, the stains of both FGF10 mRNA and FGF10 protein in HaCaT cells are reduced.